Anti-ACTL6A Antibody [ARC1433] (A305879)

Name: Anti-ACTL6A Antibody [ARC1433]
Brand: Antibodies.com
SKU: A305879

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This product is discontinued

Anti-ACTL6A Antibody [ARC1433] (A305879) has been discontinued and is no longer available.

View all Anti-ACTL6A Antibodies.

Specifications

Name

Anti-ACTL6A Antibody [ARC1433]

Description

Rabbit monoclonal [ARC1433] antibody to ACTL6A.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-290 of human ACTL6A (O96019).

Sequence

FITMQCRELFQEMNIELVPPYMIASKEAVREGSPANWKRKEKLPQVTRSWHNYMCNCVIQDFQASVLQVSDSTYDEQVAAQMPTVHYEFP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1433

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

53 kDa BRG1 associated factor A, 53 kDa BRG1-associated factor A, ACL6A_HUMAN, Actin like 6A, Actin like protein 6A, Actin related protein, Actin related protein 4, Actin related protein Baf53a, Actin-like protein 6A, Actin-related protein Baf53a, ACTL 6, ACTL 6A, ACTL6, Arp4, ARPN BETA, ArpNbeta, BAF 53A, BAF complex 53 kDa subunit, BAF53, BAF53A, BRG1 associated factor, BRG1-associated factor 53A, hArpN beta, INO80 complex subunit K, INO80K, MGC5382

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ACTL6A Antibody [ARC1433] (A305879)

Western blot analysis of extracts of various cell lines, using Anti-ACTL6A Antibody [ARC1433] (A305879) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-ACTL6A Antibody [ARC1433] (A305879)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-ACTL6A Antibody [ARC1433] (A305879) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ACTL6A Antibody [ARC1433] (A305879)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-ACTL6A Antibody [ARC1433] (A305879) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ACTL6A Antibody [ARC1433] (A305879)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-ACTL6A Antibody [ARC1433] (A305879) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.