Anti-ACTR1B Antibody [ARC3033] (A309272)

Name: Anti-ACTR1B Antibody [ARC3033]
Brand: Antibodies.com
SKU: A309272

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This product is discontinued

Anti-ACTR1B Antibody [ARC3033] (A309272) has been discontinued and is no longer available.

View all Anti-ACTR1B Antibodies.

Specifications

Name

Anti-ACTR1B Antibody [ARC3033]

Description

Rabbit monoclonal [ARC3033] antibody to ACTR1B.

Applications

WB, IHC, IP

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, IP: 1:1,000-1:5,000

Reactivity

Human, Rat

Immunogen

Recombinant protein of Human ACTR1B.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC3033

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Actin related protein 1B, Actin-related protein 1B, ACTR 1B, ACTY_HUMAN, ARP 1B, ARP1 actin related protein 1 homolog B, ARP1 actin related protein 1 homolog B centractin beta, ARP1 actin related protein 1 homolog B centractin beta (yeast), ARP1 actin related protein 1 yeast homolog B centractin beta, ARP1 yeast homolog B, ARP1B, Beta centractin, Beta-centractin, Centractin beta, Centractin beta (Yeast), CTRN 2, CTRN2, PC 3, PC3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ACTR1B Antibody [ARC3033] (A309272)

Western blot analysis of extracts of various cell lines, using Anti-ACTR1B Antibody [ARC3033] (A309272) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-ACTR1B Antibody [ARC3033] (A309272)

Western blot analysis of extracts of Rat brain, using Anti-ACTR1B Antibody [ARC3033] (A309272) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-ACTR1B Antibody [ARC3033] (A309272)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-ACTR1B Antibody [ARC3033] (A309272) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ACTR1B Antibody [ARC3033] (A309272)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-ACTR1B Antibody [ARC3033] (A309272) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.