Anti-ADFP Antibody [ARC51735] (A305635)

Name: Anti-ADFP Antibody [ARC51735]
Brand: Antibodies.com
SKU: A305635
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51735] antibody to ADFP for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ADFP Antibody [ARC51735]

Description

Rabbit monoclonal [ARC51735] antibody to ADFP.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 88-437 of human Perilipin-2 (NP_001113.2).

Sequence

DRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51735

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adipophilin, Adipose differentiation related protein, Adipose differentiation-related protein, ADRP, MGC10598, Perilipin-2, PLIN2, PLIN2_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ADFP Antibody [ARC51735] (A305635)

Western blot analysis of extracts of various cell lines, using Anti-ADFP Antibody [ARC51735] (A305635) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-ADFP Antibody [ARC51735] (A305635)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ADFP Antibody [ARC51735] (A305635) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ADFP Antibody [ARC51735] (A305635)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-ADFP Antibody [ARC51735] (A305635) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-ADFP Antibody [ARC51735] (A305635)

Immunofluorescence analysis of mouse brown adipose using Anti-ADFP Antibody [ARC51735] (A305635) at a dilution of 1:100(40x lens). DAPI was used to stain the cell nuclei (blue).