Anti-ADH5 Antibody [ARC57643] (A306952)

Name: Anti-ADH5 Antibody [ARC57643]
Brand: Antibodies.com
SKU: A306952

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This product is discontinued

Anti-ADH5 Antibody [ARC57643] (A306952) has been discontinued and is no longer available.

View all Anti-ADH5 Antibodies.

Specifications

Name

Anti-ADH5 Antibody [ARC57643]

Description

Rabbit monoclonal [ARC57643] antibody to ADH5.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5/GSNOR (NP_000662.3).

Sequence

MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC57643

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADH 3, ADHX, ADHX_HUMAN, Alcohol dehydrogenase (class III) chi polypeptide, Alcohol dehydrogenase 5, alcohol dehydrogenase 5 (class III) chi polypeptide, Alcohol dehydrogenase class 3, Alcohol dehydrogenase class chi chain, Alcohol dehydrogenase class III, Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, class III alcohol dehydrogenase 5 chi subunit, FALDH, FDH, formaldehyde dehydrogenase, Glutathione dependent formaldehyde dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, GSNOR, hydroxymethyllutathione dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ADH5 Antibody [ARC57643] (A306952)

Western blot analysis of various lysates, using Anti-ADH5 Antibody [ARC57643] (A306952) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-ADH5 Antibody [ARC57643] (A306952)

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Figure 3: Immunofluorescence - Anti-ADH5 Antibody [ARC57643] (A306952)

Immunofluorescence analysis of mouse liver using Anti-ADH5 Antibody [ARC57643] (A306952) at dilution of1:300(40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-ADH5 Antibody [ARC57643] (A306952)

Immunofluorescence analysis of rat liver using Anti-ADH5 Antibody [ARC57643] (A306952) at dilution of1:300(40x lens). DAPI was used to stain the cell nuclei (blue).

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