Anti-AK2 Antibody [ARC2916] (A305993)

Name: Anti-AK2 Antibody [ARC2916]
Brand: Antibodies.com
SKU: A305993

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This product is discontinued

Anti-AK2 Antibody [ARC2916] (A305993) has been discontinued and is no longer available.

View all Anti-AK2 Antibodies.

Specifications

Name

Anti-AK2 Antibody [ARC2916]

Description

Rabbit monoclonal [ARC2916] antibody to AK2.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human AK2 (P54819).

Sequence

GFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIPDSLLIRRITGRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKIRLQAYHTQTTPLIEY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2916

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adenylate kinase 2, adenylate kinase 2, mitochondrial, Adenylate kinase isoenzyme 2, ADK2, AK 2, ATP AMP transphosphorylase, ATP-AMP transphosphorylase 2, EC 2.7.4.3, KAD2_HUMAN, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-AK2 Antibody [ARC2916] (A305993)

Western blot analysis of extracts of various cell lines, using Anti-AK2 Antibody [ARC2916] (A305993) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-AK2 Antibody [ARC2916] (A305993)

Western blot analysis of extracts from wild type (WT) and AK2 knockout (KO) HeLa cells, using Anti-AK2 Antibody [ARC2916] (A305993) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunofluorescence - Anti-AK2 Antibody [ARC2916] (A305993)

Immunofluorescence analysis of HeLa cells using Anti-AK2 Antibody [ARC2916] (A305993) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-AK2 Antibody [ARC2916] (A305993)

Immunofluorescence analysis of HepG2 cells using Anti-AK2 Antibody [ARC2916] (A305993) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).