This Alanine Aminotransferase Activity Assay Kit is a quantitative assay kit for measuring Alanine Aminotransferase activity in animal tissues, plant tissues, cell culture (adherent or suspension cells), serum, and bacteria.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Alanine aminotransferase catalyzes formation of pyruvate, which reacts with 2,4-dinitrophenylhydrazine to produce a colored hydrazone. The increase in absorbance at 505 nm reflects ALT activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cultured cells, serum, and bacteria
Assay Time
30-60 minutes
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Standard
Storage
Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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