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Anti-Aldolase A + Aldolase B + Aldolase C Antibody [E9] (A85386)

Immunofluorescence - Anti-Aldolase C Antibody (A85386) - Antibodies.com Western Blot - Anti-Aldolase C Antibody (A85386) - Antibodies.com
This product is discontinued

Anti-Aldolase A + Aldolase B + Aldolase C Antibody [E9] (A85386) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Aldolase A + Aldolase B + Aldolase C Antibody [E9]
Description
Mouse monoclonal (E9) antibody to Aldolase A + Aldolase B + Aldolase C.
Specificity
This antibody binds to Aldolase A, Aldolase B, and Aldolase C with equal specificity. The epitope for this antibody is mapped to the peptide YKKDGADFAKWRCVLKISER, amino acids 138-157 of human Aldolase C (1XFB_A).
Applications
WB, ICC/IF, IHC
Dilutions
WB: 1:10,000, ICC/IF: 1:1,000-1:5,000, IHC: 1:1,000-1:5,000
Reactivity
Human, Rat, Mouse
Immunogen
Recombinant full-length human Aldolase C, expressed in and purified from E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
E9
Isotype
IgG1
Conjugate

Unconjugated

Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunofluorescence - Anti-Aldolase C Antibody (A85386) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Aldolase A + Aldolase B + Aldolase C Antibody [E9] (A85386)

View of mixed neuron/glial cultures stained with Anti-Aldolase C Antibody (red) and Anti-GFAP Antibody (A85307 | green). Anti-Aldolase C Antibody reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Anti-Aldolase C Antibody stains the astrocytes cell body and processes, whereas Anti-GFAP Antibody labels the intermediate filament of the cytoskeleton in subset of astrocytes.
Western Blot - Anti-Aldolase C Antibody (A85386) - Antibodies.comEnlarge Image

Western Blot - Anti-Aldolase A + Aldolase B + Aldolase C Antibody [E9] (A85386)

Blots of rat brain lysate blotted with Anti-Aldolase C Antibody. The Anti-Aldolase C Antibody binds strongly and cleanly to a band at about 40 kDa. We now know that this antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on western blots.