Anti-Aldolase C Antibody [4A9] (A85385)

Left: View of mixed neuron/glial cultures stained with Anti-Aldolase C Antibody (green) and Anti-NeuN/FOX3 Antibody (A85403 | red). Anti-Aldolase C Antibody reveals strong cytoplasmic staining in astrocytes, while Anti-Fox3/NeuN Antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain. Middle and Right: Mouse brain sections (fixed by transcardial perfusion with 4% paraformaldehyde) stained with Anti-Aldolase C Antibody (red) and Anti-Vimentin Antibody (A85421 | green). In the striatum (Middle), Anti-Aldolase C Antibody positive astrocytes are highly co-stained with Anti-Vimentin Antibody, which results in yellow to gold colors. In the cerebellum (Right), however, Anti-Aldolase C Antibody positive Purkinje cells do not express vimentin, which results in red color. Insets show a higher magnification picture of Anti-Aldolase C Antibody single labeling in red. Nuclei are labeled with DAPI (blue).

A: Immunofluorescence of rat cerebellum section stained with Anti-Aldolase C Antibody [4A9] (A85385), at a dilution of 1:1,000, in green, and co-stained with Anti-GFAP Antibody (A85419), at a dilution of 1:5,000, in red. The nuclear DNA is visualised in blue using Hoechst staining. Anti-Aldolase C Antibody [4A9] (A85385) selectively labels the perikarya and dendrites of Purkinje cells, while Anti-GFAP Antibody (A85419) stains processes of Bergman glia and astrocytic cells.

B: Immunofluorescence of E20 rat cortical culture stained with Anti-Aldolase C Antibody [4A9] (A85385), at a dilution of 1:1,000 in green, and co-stained with Anti-MAP2 Antibody (A85363), at a dilution of 1:10,000 in red. The nuclear DNA is visualised in blue using Hoechst staining. Anti-Aldolase C Antibody [4A9] (A85385) labels cytosolic protein expressed in glial cells, while Anti-MAP2 Antibody (A85363) stains dendrites and perikarya of mature neurons.

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Western blot analysis of different tissue lysates using Anti-Aldolase C Antibody [4A9] (A85385), at a dilution of 1:2,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] mouse brain, [4] cow cerebellum, and [5] pig hippocampus lysates. The single strong band at about 40 kDa corresponds to Aldolase C protein.

Blots of crude cow cerebellum homogenate blotted with Anti-Aldolase C Antibody. The Anti-Aldolase C Antibody binds strongly and cleanly to a band at about 40 kDa. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.