Anti-ALKBH1 Antibody [ARC1481] (A308238)

Name: Anti-ALKBH1 Antibody [ARC1481]
Brand: Antibodies.com
SKU: A308238

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This product is discontinued

Anti-ALKBH1 Antibody [ARC1481] (A308238) has been discontinued and is no longer available.

View all Anti-ALKBH1 Antibodies.

Specifications

Name

Anti-ALKBH1 Antibody [ARC1481]

Description

Rabbit monoclonal [ARC1481] antibody to ALKBH1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 290-389 of human ALKBH1 (Q13686).

Sequence

PRVLPNPEGEGLPHCLEAPLPAVLPRDSMVEPCSMEDWQVCASYLKTARVNMTVRQVLATDQNFPLEPIEDEKRDISTEGFCHLDDQNSEVKRARINPDS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1481

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ABH, ABH1, alkB, alkB homolog 1 histone H2A dioxygenase, AlkB, alkylation repair homolog 1 (E. coli), ALKB1_HUMAN, ALKBH, Alkylated DNA repair protein alkB homolog 1, Alkylation repair homolog 1, Alkylation repair, alkB homolog, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1, hABH

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ALKBH1 Antibody [ARC1481] (A308238)

Western blot analysis of extracts of various cell lines, using Anti-ALKBH1 Antibody [ARC1481] (A308238) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-ALKBH1 Antibody [ARC1481] (A308238)

Immunofluorescence analysis of C6 cells using Anti-ALKBH1 Antibody [ARC1481] (A308238) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-ALKBH1 Antibody [ARC1481] (A308238)

Immunofluorescence analysis of NIH/3T3 cells using Anti-ALKBH1 Antibody [ARC1481] (A308238) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-ALKBH1 Antibody [ARC1481] (A308238)

Immunofluorescence analysis of U2OS cells using Anti-ALKBH1 Antibody [ARC1481] (A308238) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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