Anti-alpha 1 Fetoprotein Antibody (A81166)

Name: Anti-alpha 1 Fetoprotein Antibody
Brand: Antibodies.com
SKU: A81166
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to alpha 1 Fetoprotein for WB and ICC/IF.

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$40 to United States

Lead Time

5-8 Business Days

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Highlighted products for Anti-alpha 1 Fetoprotein Antibody

A85533

12 Citations

Anti-alpha Fetoprotein Antibody [AFP-01]

Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.

A85533

12 Citations

Anti-alpha Fetoprotein Antibody [AFP-01]

Mouse monoclonal [AFP-01] antibody to alpha Fetoprotein for IP, ELISA, WB and ICC.

Specifications

Name

Anti-alpha 1 Fetoprotein Antibody

Description

Rabbit polyclonal antibody to alpha 1 Fetoprotein.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 395-609 of Alpha-Fetoprotein (AFP) (NP_001125.1).

Sequence

DKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA_HUMAN, Hereditary persistence of alpha fetoprotein, HPAFP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-alpha 1 Fetoprotein Antibody (A81166)

Western blot analysis of extracts of mouse liver, using Anti-alpha 1 Fetoprotein Antibody (A81166) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunofluorescence - Anti-alpha 1 Fetoprotein Antibody (A81166)

Immunofluorescence analysis of HepG2 cells (upper left) and LO2 cells (negative sample control, (upper right) using Anti-alpha 1 Fetoprotein Antibody (A81166), at a dilution of 1:100, (Red). DAPI was used to stain the cell nuclei (blue).

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