Anti-alpha Tubulin Antibody [YL1/2] (HRP) (A282062)

Anti-alpha Tubulin Antibody [YL1/2] (HRP)

Rat monoclonal [YL1/2] antibody to alpha Tubulin (HRP).

This antibody recognises the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987). Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.

ELISA, WB, IHC-Fr

WB: 1:100 - 1:1,000, MCA77P is suitable for use as a loading control.

S. cerevisiae, Ashbya, Birds, Echinoderm, Human, Mouse, Dog, Rat, Pig, Drosophila, Plants, Amphibia, Saccharomyces, Pleurobrachia, Caenorhabditis, Dictyostelium discoideum, Xenopus, Pig-tailed macaque, Clytia sp., Arabidopsis, Strongylocentrotus purpuratus, Dendraster excentricus, Trypanosoma brucei, Potorous tridactylis, Bovine, Hemicentrotus pulcherrimus, Potato, Bombyx mori, Rhodnius prolixus, Beroe abyssicola, Candida sp.

This antibody does not cross react with Nephrotoma suturalis

Yeast tubulin.

Rat

Monoclonal

IgG2a

HRP

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.01% Thiomersal and HRP Stabiliser (Proprietary stabiliser based on a TRIS buffer, <15ppm CMIT/MIT).

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti tubulin alpha antibody, clone YL1/2 recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin (Burns 1987).Rat anti tubulin alpha antibody, clone YL1/2 is routinely tested in ELISA on tubulin.

Alpha-tubulin 1, ALS22, B ALPHA 1, bA408E5.3, H2 ALPHA, Hum a tub1, Hum a tub2, LIS3, MGC171407, MGC55332, TBA4A_HUMAN, Testis-specific alpha-tubulin, TUBA1, TUBA1A, tuba1l, TUBA2, TUBA3, TUBA3C, Tuba4a, Tubulin alpha, Tubulin alpha 1 chain, Tubulin alpha-1 chain, tubulin alpha-1B chain, Tubulin alpha-4A chain, Tubulin H2-alpha, Tubulin, alpha 1 (testis specific), tubulin, alpha 1, like, Tubulin, alpha 1a, Tubulin, alpha 3c, Tubulin, alpha 4a, Tubulin, alpha, testis-specific, Tubulin, alpha-1

This product is for research use only. It is not intended for diagnostic or therapeutic use.