Anti-Amphiphysin Antibody (A11829)

Name: Anti-Amphiphysin Antibody
Brand: Antibodies.com
SKU: A11829
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Amphiphysin for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Amphiphysin Antibody

Description

Rabbit polyclonal antibody to Amphiphysin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human AMPH (NP_001626.1).

Sequence

MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

125 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Amp, AMPH, AMPH 1, AMPH1, Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen), Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen), Amphiphysin I, AMPH_HUMAN, CG8604, Damp, dAmph, DmelCG8604, HGNC:471, MGC49429, Stiff man syndrome with breast cancer 128kD autoantigen, Stiff man syndrome with breast cancer 128kDa autoantigen

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Amphiphysin Antibody (A11829)

Western blot analysis of extracts of various cell lines, using Anti-Amphiphysin Antibody (A11829) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-Amphiphysin Antibody (A11829)

Immunofluorescence analysis of U-251 MG cells using Anti-Amphiphysin Antibody (A11829) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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