Anti-AMPK alpha 1 + AMPK alpha 2 Antibody [84.1] (A278763)

Anti-AMPK alpha 1 + AMPK alpha 2 Antibody [84.1]

Mouse monoclonal [84.1] antibody to AMPK alpha 1 + AMPK alpha 2.

This antibody recognises both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilized by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. AMPK functions as a suppressor of cell proliferation of both malignant and normal cells.

WB, IHC-Fr, ELISA, IF, IP

WB: 1:100 - 1:1,000

Drosophila, Vertebrates, Human, Mouse, Rat

A peptide corresponding to 29 amino acids within the N-terminal catalytic domain of Drosophila AMPK alpha.

Mouse

Monoclonal

84.1

IgG2a

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Approximately 63 kDa in rat brain cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti AMPK Alpha 1/2 antibody, clone 84.1 recognises both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilized by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels.AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. AMPK functions as a suppressor of cell proliferation of both malignant and normal cells (Motoshima et al. 2006).

5''-AMP-activated protein kinase catalytic subunit alpha-1, AAPK1_HUMAN, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK, AMPK alpha 1 chain, AMPK alpha 2 chain, AMPK subunit alpha-1, AMPK1, AMPK2, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA, PRKAA1, PRKAA2, Protein kinase AMP activated alpha 1 catalytic subunit, Protein kinase AMP activated alpha 2 catalytic subunit, Tau-protein kinase PRKAA1