Anti-APC Antibody [ARC53932] (A308377)

Name: Anti-APC Antibody [ARC53932]
Brand: Antibodies.com
SKU: A308377

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This product is discontinued

Anti-APC Antibody [ARC53932] (A308377) has been discontinued and is no longer available.

View all Anti-APC Antibodies.

Specifications

Name

Anti-APC Antibody [ARC53932]

Description

Rabbit monoclonal [ARC53932] antibody to APC.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2600-2760 of human APC (NP_000029.2).

Sequence

QSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAESKTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLENRLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53932

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adenomatous Polyposis Coli, Adenomatous polyposis coli protein, APC_HUMAN, CC1, Deleted in polyposis 2.5, DP2, DP2.5, DP3, FAP, FPC, GS, Protein APC

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-APC Antibody [ARC53932] (A308377)

Western blot analysis of extracts of various cell lines, using Anti-APC Antibody [ARC53932] (A308377) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-APC Antibody [ARC53932] (A308377)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-APC Antibody [ARC53932] (A308377) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-APC Antibody [ARC53932] (A308377)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-APC Antibody [ARC53932] (A308377) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-APC Antibody [ARC53932] (A308377)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-APC Antibody [ARC53932] (A308377) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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