Anti-ARHGAP4 Antibody (A308756)

Name: Anti-ARHGAP4 Antibody
Brand: Antibodies.com
SKU: A308756
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ARHGAP4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ARHGAP4 Antibody

Description

Rabbit polyclonal antibody to ARHGAP4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human ARHGAP4 (NP_001158213.1).

Sequence

SPEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSPSPGPRSPKAPPSSRLGRNKGFSRGPGAPASPSASHPQGLDTTPKPH

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

105 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ARHGAP 4, C1, GTPase-activating protein, Rho, 4, KIAA0131, OTTHUMP00000026004, p115, RGC 1, RGC1, Rho GAP hematopoietic protein C1, Rho GTPase activating protein 4, Rho type GTPase activating protein 4, RhoGAP 4, RhoGAP4, SrGAP4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ARHGAP4 Antibody (A308756)

Western blot analysis of extracts of Jurkat cells, using Anti-ARHGAP4 Antibody (A308756) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-ARHGAP4 Antibody (A308756)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-ARHGAP4 Antibody (A308756) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ARHGAP4 Antibody (A308756)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-ARHGAP4 Antibody (A308756) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ARHGAP4 Antibody (A308756)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-ARHGAP4 Antibody (A308756) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.