Anti-ARPC2 Antibody [ARC2558] (A305917)

Name: Anti-ARPC2 Antibody [ARC2558]
Brand: Antibodies.com
SKU: A305917
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2558] antibody to ARPC2 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ARPC2 Antibody [ARC2558]

Description

Rabbit monoclonal [ARC2558] antibody to ARPC2.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ARPC2 (O15144).

Sequence

FSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2558

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Actin related protein 2/3 complex subunit 2, Actin related protein 2/3 complex subunit 2 34kDa, Actin-related protein 2/3 complex subunit 2, ARC 34, ARC34, Arp2/3 complex 34 kDa subunit, ARP2/3 protein compex subunit 34, ARPC 2, ARPC2_HUMAN, p34 Arc, p34-ARC, p34Arc, PNAS 139, PNAS139, PRO 2446, PRO2446

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ARPC2 Antibody [ARC2558] (A305917)

Western blot analysis of extracts of various cell lines, using Anti-ARPC2 Antibody [ARC2558] (A305917) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-ARPC2 Antibody [ARC2558] (A305917)

Immunofluorescence analysis of NIH/3T3 cells using Anti-ARPC2 Antibody [ARC2558] (A305917) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-ARPC2 Antibody [ARC2558] (A305917)

Immunofluorescence analysis of PC-12 cells using Anti-ARPC2 Antibody [ARC2558] (A305917) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-ARPC2 Antibody [ARC2558] (A305917)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-ARPC2 Antibody [ARC2558] (A305917). This Western blot was performed on the immunoprecipitate using Anti-ARPC2 Antibody [ARC2558] (A305917) at a dilution of 1:1000.

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