Rabbit polyclonal antibody to Arrestin 1 (Phospho-Ser412)
Specificity
The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
48 kDa protein, Arrestin, ARRS_HUMAN, Retinal S antigen, Retinal S antigen (48 KDa protein), Retinal S-antigen, Rod photoreceptor arrestin, RP47, S antigen, S antigen retina and pineal gland (arrestin), S arrestin, S-AG, S-arrestin, SAG
Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody #11654.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody #11654 (left)or the same antibody preincubated with blocking peptide (right).
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