Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558)

Name: Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212]
Brand: Antibodies.com
SKU: A306558

Enlarge Image

This product is discontinued

Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558) has been discontinued and is no longer available.

View all Anti-Aryl hydrocarbon Receptor Antibodies.

Specifications

Name

Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212]

Description

Rabbit Oligoclonal [ARC53211 + ARC53212] antibody to Aryl hydrocarbon Receptor.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 619-848 of human AHR Rabbit PolymAb® (NP_001612.1).

Sequence

LEQQQQHHQKQVVVEPQQQLCQKMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYPMGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC53211 + ARC53212

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

105 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ah receptor, AhR, AHR_HUMAN, Aromatic hydrocarbon receptor, Aryl hydrocarbon receptor precursor, bHLHe76, Class E basic helix loop helix protein 76, Class E basic helix-loop-helix protein 76, HGNC:348

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558)

Western blot analysis of PC-3, using Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Show more images

Publishing research using Anti-Aryl hydrocarbon Receptor Antibody [ARC53211 + ARC53212] (A306558)? Please let us know so that we can list the citation on this page.