Anti-ASIC1 Antibody [S271-44] (A305010)

Name: Anti-ASIC1 Antibody [S271-44]
Brand: Antibodies.com
SKU: A305010
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S271-44] antibody to ASIC1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ASIC1 Antibody [S271-44]

Description

Mouse monoclonal [S271-44] antibody to ASIC1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1 .

Host

Mouse

Clonality

Monoclonal

Clone ID

S271-44

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACCN2, acid-sensing (proton-gated) ion channel 1, acid-sensing ion channel 1, acid-sensing ion channel 1a protein, amiloride-sensitive cation channel 2, neuronal, ASIC, BNaC2, brain sodium channel 2, Cation channel, amiloride-sensitive, neuronal, 2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-ASIC1 Antibody [S271-44] (A305010)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-ASIC1 Antibody [S271-44] (A305010), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ASIC1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-ASIC1 Antibody [S271-44] (A305010)

Western blot analysis of rat brain lysates showing detection of ASIC1 protein using Anti-ASIC1 Antibody [S271-44] (A305010) at 1:100, 1:250, 1:500, and 1:1,000.

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Figure 3: Immunofluorescence - Anti-ASIC1 Antibody [S271-44] (A305010)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ASIC1 Antibody [S271-44] (A305010), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ASIC1 Antibody. (D) Composite.

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