Anti-Aspartate Aminotransferase Antibody (A14997)

Name: Anti-Aspartate Aminotransferase Antibody
Brand: Antibodies.com
SKU: A14997
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Aspartate Aminotransferase for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Aspartate Aminotransferase Antibody

Description

Rabbit polyclonal antibody to Aspartate Aminotransferase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:10-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).

Sequence

FVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AATC_HUMAN, Aspartate aminotransferase 1, Aspartate aminotransferase cytoplasmic, Aspartate aminotransferase cytosolic, ASTQTL1, cAspAT, cCAT, cysteine aminotransferase, cytoplasmic, cysteine transaminase, cytoplasmic, cytoplasmic, ec 2.6.1.1, GIG 18, GIG18, Glutamate oxaloacetate transaminase 1, Glutamate oxaloacetate transaminase soluble, Glutamic oxaloacetic transaminase 1 soluble, GOT 1, GOT1, Growth inhibiting protein 18, SGOT, Transaminase A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Aspartate Aminotransferase Antibody (A14997)

Western blot analysis of extracts of various cell lines, using Anti-Aspartate Aminotransferase Antibody (A14997) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Aspartate Aminotransferase Antibody (A14997)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Aspartate Aminotransferase Antibody (A14997) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Aspartate Aminotransferase Antibody (A14997)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Aspartate Aminotransferase Antibody (A14997) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Aspartate Aminotransferase Antibody (A14997)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Aspartate Aminotransferase Antibody (A14997) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.