Anti-ATF-4 Antibody (A308278)

Name: Anti-ATF-4 Antibody
Brand: Antibodies.com
SKU: A308278
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to ATF-4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-ATF-4 Antibody

Description

Rabbit polyclonal antibody to ATF-4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 260-351 of human ATF4 (NP_877962.1).

Sequence

PYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Activating transcription factor 4, ATF 4, ATF4, ATF4 protein, ATF4_HUMAN, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB 2, CREB-2, CREB2, Cyclic AMP dependent transcription factor ATF 4, Cyclic AMP response element binding protein 2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2, DNA binding protein TAXREB67, DNA-binding protein TAXREB67, Tax Responsive Enhancer Element B67, Tax-responsive enhancer element-binding protein 67, TaxREB67, TXREB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-ATF-4 Antibody (A308278)

Western blot analysis of extracts of C2C12 cells, using Anti-ATF-4 Antibody (A308278) at 1:500 dilution. C2C12 cells were treated by tunicamycin (2 µg/ml) for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-ATF-4 Antibody (A308278)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-ATF-4 Antibody (A308278) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ATF-4 Antibody (A308278)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-ATF-4 Antibody (A308278) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-ATF-4 Antibody (A308278)

Immunofluorescence analysis of human breast cancer cells using Anti-ATF-4 Antibody (A308278) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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