Anti-ATG12 Antibody [ARC2663] (A309284)

Name: Anti-ATG12 Antibody [ARC2663]
Brand: Antibodies.com
SKU: A309284

Enlarge Image

This product is discontinued

Anti-ATG12 Antibody [ARC2663] (A309284) has been discontinued and is no longer available.

View all Anti-ATG12 Antibodies.

Specifications

Name

Anti-ATG12 Antibody [ARC2663]

Description

Rabbit monoclonal [ARC2663] antibody to ATG12.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human ATG12.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2663

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apg12, APG12-like, APG12L, ATG12 autophagy related 12 homolog, ATG12 autophagy related 12 homolog (S. cerevisiae), ATG12_HUMAN, Autophagy 12, Autophagy-related protein 12, FBR93, HAPG12, Ubiquitin-like protein ATG12

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-ATG12 Antibody [ARC2663] (A309284)

Western blot analysis of various lysates, using Anti-ATG12 Antibody [ARC2663] (A309284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-ATG12 Antibody [ARC2663] (A309284)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ATG12 Antibody [ARC2663] (A309284) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Western Blot - Anti-ATG12 Antibody [ARC2663] (A309284)

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-ATG12 Antibody [ARC2663] (A309284). This Western blot was performed on the immunoprecipitate using Anti-ATG12 Antibody [ARC2663] (A309284) at a dilution of 1:500.

Publishing research using Anti-ATG12 Antibody [ARC2663] (A309284)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-ATG12 Antibody [ARC2663] (A309284)

(2)

A121605

1 Citation