Anti-ATG4B Antibody (A304916)

Name: Anti-ATG4B Antibody
Brand: Antibodies.com
SKU: A304916
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to ATG4B for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-ATG4B Antibody

Description

Rabbit polyclonal antibody to ATG4B.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse

Immunogen

Synthetic peptide from the N-terminal of Human ATG4B (aa. 62-73).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

APG4 autophagy 4 homolog B, APG4B, ATG4 autophagy related 4 homolog B (S. cerevisiae), ATG4B_HUMAN, AUT like 1 cysteine endopeptidase, AUT-like 1 cysteine endopeptidase, AUTL1, Autophagin 1, Autophagin-1, Autophagy related 4B cysteine peptidase, Autophagy related cysteine endopeptidase 1, Autophagy related protein 4 homolog B, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, Cysteine protease ATG4B, hAPG4B, MGC1353

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Immunofluorescence - Anti-ATG4B Antibody (A304916)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATG4B Antibody (A304916), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4B Antibody (D) Composite.

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Figure 2: Western Blot - Anti-ATG4B Antibody (A304916)

Western blot analysis of mouse brain cell lysates showing detection of 44.3 kDa ATG4B protein using Anti-ATG4B Antibody (A304916) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 44.3 kDa.

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