Anti-ATG7 Antibody [ARC2631] (A306024)

Name: Anti-ATG7 Antibody [ARC2631]
Brand: Antibodies.com
SKU: A306024

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This product is discontinued

Anti-ATG7 Antibody [ARC2631] (A306024) has been discontinued and is no longer available.

View all Anti-ATG7 Antibodies.

Specifications

Name

Anti-ATG7 Antibody [ARC2631]

Description

Rabbit monoclonal [ARC2631] antibody to ATG7.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-703 of human ATG7 (O95352).

Sequence

GGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2631

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1810013K23Rik, Apg 7, Apg7, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7 like, APG7, S. cerevisiae, homolog of, APG7-like, APG7L, ATG 7, ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), Atg7l, ATG7_HUMAN, Autophagy 7, S. cerevisiae, homolog of, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, GSA7, hAGP7, Ubiquitin activating enzyme E1 like protein, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-ATG7 Antibody [ARC2631] (A306024)

Western blot analysis of extracts of HepG2 cells, using Anti-ATG7 Antibody [ARC2631] (A306024) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-ATG7 Antibody [ARC2631] (A306024)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ATG7 Antibody [ARC2631] (A306024) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-ATG7 Antibody [ARC2631] (A306024)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-ATG7 Antibody [ARC2631] (A306024). This Western blot was performed on the immunoprecipitate using Anti-ATG7 Antibody [ARC2631] (A306024) at a dilution of 1:500.

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