ATP1A1 (phospho Ser23) Cell Based ELISA Kit (A102792)

ATP1A1 (phospho Ser23) Cell Based ELISA Kit

Cell-based (qualitative)

This ATP1A1 (phospho Ser23) Cell Based ELISA Kit allows for the detection of ATP1A1 (phospho Ser23) and the effects that certain stimulation conditions have on ATP1A1 (phospho Ser23) expression in different cell lines. Qualitative determination of ATP1A1 (phospho Ser23) concentration is achieved by an indirect ELISA format. In essence, the ATP1A1 (phospho Ser23) is captured by Anti-ATP1A1 (phospho Ser23) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this ATP1A1 (phospho Ser23) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-ATP1A1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Microplate

Colorimetric

Adherent cells and suspension cells.

4 hours 30 minutes

Rat

Store at + 4°C. Stable for 6 months.

adenosinetriphosphatase, AT1A1_HUMAN, ATP1A4, ATP1AL2, ATP1B, ATP1B1, ATPase Na+/K+ transporting alpha, ATPase Na+/K+ transporting alpha 1 polypeptide, ATPase Na+/K+ transporting alpha 4 polypeptide, ATPase Na+/K+ transporting beta 1 polypeptide, ATPase, Na+/K+ transporting, alpha polypeptide-like 2, ATPase, Na+/K+ transporting, beta 1 polypeptide, Beta 1-subunit of Na(+),K(+)-ATPase, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase alpha-4 subunit, Na+, K+ ATPase alpha subunit, Na+/K+ ATPase 1, Na+/K+ ATPase 4, Na+/K+ ATPase, alpha-D polypeptide, Na, K-ATPase beta-1 polypeptide, Na, K-ATPase, alpha-A catalytic polypeptide, Na,K-ATPase catalytic subunit alpha-A protein, Na,K-ATPase subunit alpha-C, polypeptide-like 2, Sodium Potassium ATPase, Sodium pump 1, sodium pump 4, Sodium pump subunit alpha-1, sodium pump subunit alpha-4, sodium pump subunit beta-1, sodium-potassium ATPase catalytic subunit alpha-1, sodium-potassium ATPase catalytic subunit alpha-4, sodium-potassium ATPase subunit beta 1 (non-catalytic), sodium-potassium ATPase, alpha 4 polypeptide, sodium-potassium-ATPase, alpha 1 polypeptide, Sodium/potassium transporting ATPase alpha 1 chain, Sodium/potassium transporting ATPase subunit beta 1, sodium/potassium-dependent ATPase beta-1 subunit, sodium/potassium-transporting ATPase alpha-4 chain, sodium/potassium-transporting ATPase beta-1 chain, Sodium/potassium-transporting ATPase subunit alpha-1, sodium/potassium-transporting ATPase subunit alpha-4