Anti-ATPB Antibody [ARC53533] (A81028)

Name: Anti-ATPB Antibody [ARC53533]
Brand: Antibodies.com
SKU: A81028
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53533] antibody to ATPB for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Mitochondrial Marker

Specifications

Name

Anti-ATPB Antibody [ARC53533]

Description

Rabbit monoclonal [ARC53533] antibody to ATPB.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATPB (NP_001677.2).

Sequence

YSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53533

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

57 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATP 5B, ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide, ATP synthase subunit beta mitochondrial, ATP synthase subunit beta, mitochondrial, atp5b, ATPB_HUMAN, ATPMB, ATPSB, Epididymis secretory protein Li 271, HEL-S-271, Mitochondrial ATP synthase beta subunit, Mitochondrial ATP Synthase Subunit Beta, Mitochondrial ATP synthetase beta subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-ATPB Antibody [ARC53533] (A81028)

Western blot analysis of extracts of various cell lines, using Anti-ATPB Antibody [ARC53533] (A81028) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-ATPB Antibody [ARC53533] (A81028)

Western blot analysis of extracts of various cell lines, using Anti-ATPB Antibody [ARC53533] (A81028) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-ATPB Antibody [ARC53533] (A81028)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-ATPB Antibody [ARC53533] (A81028) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATPB Antibody [ARC53533] (A81028)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-ATPB Antibody [ARC53533] (A81028) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.