Anti-ATR Antibody [ARC53816] (A306036)

Name: Anti-ATR Antibody [ARC53816]
Brand: Antibodies.com
SKU: A306036
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53816] antibody to ATR for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-ATR Antibody [ARC53816]

Description

Rabbit monoclonal [ARC53816] antibody to ATR.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2).

Sequence

GISPK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53816

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

301 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Ataxia telangiectasia and Rad3 related, Ataxia telangiectasia and Rad3-related protein, ATR_HUMAN, FCTCS, FRAP Related Protein 1, FRAP-related protein 1, FRP1, MEC1, MEC1 mitosis entry checkpoint 1 homolog, Protein kinase ATR, RAC3, Rad3 related protein, SCKL, SCKL1, Serine/threonine protein kinase ATR, Serine/threonine-protein kinase ATR

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-ATR Antibody [ARC53816] (A306036)

Western blot analysis of 293F, using Anti-ATR Antibody [ARC53816] (A306036) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-ATR Antibody [ARC53816] (A306036)

Immunohistochemistry analysis of paraffin-embedded human cervix cancer tissue using Anti-ATR Antibody [ARC53816] (A306036) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-ATR Antibody [ARC53816] (A306036)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-ATR Antibody [ARC53816] (A306036) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-ATR Antibody [ARC53816] (A306036)

Immunohistochemistry analysis of paraffin-embedded rat colon using Anti-ATR Antibody [ARC53816] (A306036) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.