Rabbit polyclonal antibody to ATRIP (Phospho-Ser224)
Specificity
The antibody detects endogenous levels of ATRIP only when phosphorylated at serine 224.
Applications
WB, IHC
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 224 (H-V-S(p)-P-R) derived from Human ATRIP.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
AGS1, ATIP, ATM and Rad3 related interacting protein, ATM and Rad3-related-interacting protein, ATR interacting protein, ATR-interacting protein, ATRIP_HUMAN, DKFZp762J2115, FLJ12343, MGC20625, MGC21482, MGC26740
Western blot analysis of extracts from 292 cells, treated with UV (15mins), using ATRIP (Phospho-Ser224) antibody #12130. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using ATRIP (Phospho-Ser224) Antibody #12130. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ATRIP (Phospho-Ser224) antibody #12130. The picture on the right is treated with the synthesized peptide.
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