Anti-Aurora B Antibody [ARC50892] (A306973)

Name: Anti-Aurora B Antibody [ARC50892]
Brand: Antibodies.com
SKU: A306973

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This product is discontinued

Anti-Aurora B Antibody [ARC50892] (A306973) has been discontinued and is no longer available.

View all Anti-Aurora B Antibodies.

Specifications

Name

Anti-Aurora B Antibody [ARC50892]

Description

Rabbit monoclonal [ARC50892] antibody to Aurora B.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Aurora B (NP_004208.2).

Sequence

MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50892

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AIK2, AIM-1, AIM1, ARK-2, ARK2, AurB, AURKB, AURKB_HUMAN, Aurora 1, Aurora and Ipl1 like midbody associated protein 1, Aurora kinase B, Aurora related kinase 2, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1 related kinase 2, Aurora/IPL1-related kinase 2, IPL1, PPP1R48, Protein phosphatase 1 regulatory subunit 48, Serine/theronine kinase 12, Serine/threonine protein kinase 12, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK-1, STK1, STK12, STK5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Aurora B Antibody [ARC50892] (A306973)

Western blot analysis of extracts of Jurkat cells, using Anti-Aurora B Antibody [ARC50892] (A306973) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Aurora B Antibody [ARC50892] (A306973)

Western blot analysis of extracts of various cell lines, using Anti-Aurora B Antibody [ARC50892] (A306973) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-Aurora B Antibody [ARC50892] (A306973)

Western blot analysis of extracts from wild type(WT) and Aurora B knockdown (KD) 293T cells, using Anti-Aurora B Antibody [ARC50892] (A306973) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunofluorescence - Anti-Aurora B Antibody [ARC50892] (A306973)

Immunofluorescence analysis of C6 using Anti-Aurora B Antibody [ARC50892] (A306973) at a dilution of 1:1,000 (40x lens). DAPI was used to stain the cell nuclei (blue).

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