Anti-Axl Antibody [ARC53013] (A305702)

Name: Anti-Axl Antibody [ARC53013]
Brand: Antibodies.com
SKU: A305702

Enlarge Image

This product is discontinued

Anti-Axl Antibody [ARC53013] (A305702) has been discontinued and is no longer available.

View all Anti-Axl Antibodies.

Specifications

Name

Anti-Axl Antibody [ARC53013]

Description

Rabbit monoclonal [ARC53013] antibody to Axl.

Applications

WB, Flow Cytometry

Dilutions

WB: 1:1,000-1:5,000, Flow Cytometry: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-449 of human Axl (NP_068713.2).

Sequence

EESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53013

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

138 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adhesion related kinase, AI323647 , Ark , AXL oncogene, AXL receptor tyrosine kinase, AXL transforming gene , AXL transforming sequence/gene , EC 2.7.10.1, JTK11, Oncogene AXL, Tyro7 , Tyrosine protein kinase receptor UFO , Tyrosine-protein kinase receptor UFO, UFO, UFO_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-Axl Antibody [ARC53013] (A305702)

Western blot analysis of various lysates, using Anti-Axl Antibody [ARC53013] (A305702) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 2: Flow Cytometry - Anti-Axl Antibody [ARC53013] (A305702)

Flow cytometry analysis of U-251 MG cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-Axl Antibody [ARC53013] (A305702), (10 µg/ml orange line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained U-251 MG cells, used as blank control (red line).

Enlarge Image

Figure 3: Flow Cytometry - Anti-Axl Antibody [ARC53013] (A305702)

Flow cytometry analysis of Hela cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-Axl Antibody [ARC53013] (A305702), (10 µg/ml orange line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained Hela cells, used as blank control (red line).

Enlarge Image

Figure 4: Flow Cytometry - Anti-Axl Antibody [ARC53013] (A305702)

Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-Axl Antibody [ARC53013] (A305702), (2.5 µg/ml orange line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained Jurkat cells, used as blank control (red line).

Publishing research using Anti-Axl Antibody [ARC53013] (A305702)? Please let us know so that we can list the citation on this page.