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Anti-Baf180 Antibody [ARC1820] (A309071)

Western Blot - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.com Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.com Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.com Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC1820] antibody to Baf180 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Baf180 Antibody [ARC1820]
Description
Rabbit monoclonal [ARC1820] antibody to Baf180.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 530-680 of human PBRM1 (Q86U86).
Sequence
VLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1820
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BRG1-associated factor 180, CG11375, hPB1, MGC156155, MGC156156, OTTHUMP00000202149, OTTHUMP00000202150, OTTHUMP00000202151, OTTHUMP00000202153, OTTHUMP00000202163, PB1, PB1_HUMAN, Pbrm1, polybromo 1, Polybromo-1D, Protein polybromo-1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Baf180 Antibody [ARC1820] (A309071)
Western blot analysis of extracts of various cell lines, using Anti-Baf180 Antibody [ARC1820] (A309071) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071)
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Baf180 Antibody [ARC1820] (A309071) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Baf180 Antibody [ARC1820] (A309071) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Baf180 Antibody [ARC1820] (A309071)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Baf180 Antibody [ARC1820] (A309071) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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