Anti-BAF53b Antibody (A15546)

Name: Anti-BAF53b Antibody
Brand: Antibodies.com
SKU: A15546
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to BAF53b for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-BAF53b Antibody

Description

Rabbit polyclonal antibody to BAF53b.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6B (NP_057272.1).

Sequence

MSGGVYGGDEVGALVFDIGSFSVRAGYAGEDCPKADFPTTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHVPRDGAEVMSPLKNGMIEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAPWNTRAKREKLTELMFEQYNIPAFFLCKTAVLTAFANGRSTGLVLDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFISMQCRELFQEMAIDIIPPYMIAAKEPVREGAPPNWKKKEKLPQVSKSWHNYMCNEVIQDFQASVLQVSDSPYDEQVAAQMP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

47 - 55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

53 kDa BRG1 associated factor B, 53 kDa BRG1-associated factor B, ACL 6B, ACL6B, ACL6B_HUMAN, Actin like 6, Actin like protein 6B, Actin like protein6B, Actin related protein, Actin related protein Baf53b, actin-like 6B, Actin-like protein 6B, Actin-related protein Baf53b, Actl 6, ACTL 6B, Actl6, ACTL6 B, Actl6b, Arp Na, ArpN a, ArpN alpha, ArpNa, ArpNalpha, Baf 53b, BRG1-associated factor 53B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-BAF53b Antibody (A15546)

Western blot analysis of extracts of various cell lines, using Anti-BAF53b Antibody (A15546) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 40s.

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Figure 2: Immunohistochemistry - Anti-BAF53b Antibody (A15546)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-BAF53b Antibody (A15546) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-BAF53b Antibody (A15546)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using Anti-BAF53b Antibody (A15546) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-BAF53b Antibody (A15546)

Immunohistochemistry analysis of paraffin-embedded mouse jejunum using Anti-BAF53b Antibody (A15546) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.