Anti-Bag1 Antibody [ARC2791] (A309173)

Name: Anti-Bag1 Antibody [ARC2791]
Brand: Antibodies.com
SKU: A309173
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2791] antibody to Bag1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Bag1 Antibody [ARC2791]

Description

Rabbit monoclonal [ARC2791] antibody to Bag1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Bag1 (Q99933).

Sequence

WSEEATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2791

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

33 kDa / 46 kDa / 52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Bag 1, BAG family molecular chaperone regulator 1, BAG-1, BAG1_HUMAN, BCL 2 associated athanogene, BCL 2 Associated Athanogene 1, BCL 2 binding athanogene 1, Bcl-2-associated athanogene 1, BCL2 associated athanogene, BCL2 associated athanogene 1, Glucocorticoid receptor-associated protein RAP46, HAP1, Haptoglobin, RAP 46, RAP46

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Bag1 Antibody [ARC2791] (A309173)

Western blot analysis of extracts of various cell lines, using Anti-Bag1 Antibody [ARC2791] (A309173) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Bag1 Antibody [ARC2791] (A309173)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Bag1 Antibody [ARC2791] (A309173) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Bag1 Antibody [ARC2791] (A309173)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Bag1 Antibody [ARC2791] (A309173) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Bag1 Antibody [ARC2791] (A309173)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Bag1 Antibody [ARC2791] (A309173) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.