Anti-Bak Antibody (A12537)

Name: Anti-Bak Antibody
Brand: Antibodies.com
SKU: A12537
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Bak for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Bak Antibody

Description

Rabbit polyclonal antibody to Bak.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bak (NP_001179.1).

Sequence

MASGQGPGPPRQECGEPALPSASEEQVAQDTEEVFRSYVFYRHQQEQEAEGVAAPADPEMVTLPLQPSSTMGQVGRQLAIIGDDINRRYDSEFQTMLQHL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator BAK, BAK like, Bak NT, Bak1, BAK_HUMAN, Bcl 2 homologous antagonist/killer, Bcl 2 like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, BCL2 antagonist/killer 1, Bcl2 like 7 Protein, Bcl2-L-7, BCL2L7, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Bak Antibody (A12537)

Western blot analysis of extracts of THP-1 cells, using Anti-Bak Antibody (A12537) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-Bak Antibody (A12537)

Immunofluorescence analysis of C6 cells using Anti-Bak Antibody (A12537) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Bak Antibody (A12537)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Bak Antibody (A12537) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-Bak Antibody (A12537)

Immunoprecipitation analysis of 300µg extracts of THP-1 cells using 3µg of Anti-Bak Antibody (A12537). This Western blot was performed on the immunoprecipitate using Anti-Bak Antibody (A12537) at a dilution of 1:1000.

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