Anti-BCAR1 Antibody (A305776)

Name: Anti-BCAR1 Antibody
Brand: Antibodies.com
SKU: A305776
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to BCAR1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-BCAR1 Antibody

Description

Rabbit polyclonal antibody to BCAR1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 495-612 of human BCAR1 (NP_055382.2).

Sequence

EPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQALDAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCAR 1, BCAR1_HUMAN, Breast cancer anti estrogen resistance 1, Breast cancer anti estrogen resistance 1 protein, Breast cancer anti-estrogen resistance protein 1, CAS, Cas scaffolding protein family member 1, CAS1, Cass1, Crk associated substrate, Crk associated substrate p130Cas, CRK-associated substrate, CRKAS, FLJ12176, FLJ45059, p130cas

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-BCAR1 Antibody (A305776)

Western blot analysis of extracts of Mouse brain, using Anti-BCAR1 Antibody (A305776) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-BCAR1 Antibody (A305776)

Western blot analysis of extracts of HeLa cells, using Anti-BCAR1 Antibody (A305776) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-BCAR1 Antibody (A305776)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-BCAR1 Antibody (A305776) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-BCAR1 Antibody (A305776)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-BCAR1 Antibody (A305776) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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