Anti-Bcl-X Antibody [2H12] (A249886)

Mouse monoclonal [2H12] antibody to Bcl-X for Flow Cytometry, IF, WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Bcl-X Antibody [2H12]
Description
Mouse monoclonal [2H12] antibody to Bcl-X.
Specificity
This antibody recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat, Pig
Immunogen
Synthetic peptide corresponding to amino acids 3-14 of human Bcl-X protein.
Sequence
Cys-QSNRELVVDFLS
Host
Mouse
Clonality
Monoclonal
Clone ID
2H12
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
27 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Bcl-X Antibody [2H12] - BSA and Azide free (A253066).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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