Anti-Bcl-XL Antibody (A12541)

Name: Anti-Bcl-XL Antibody
Brand: Antibodies.com
SKU: A12541
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Bcl-XL for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-Bcl-XL Antibody

Description

Rabbit polyclonal antibody to Bcl-XL.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human Bcl-XL (NP_612815.1).

Sequence

MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Apoptosis regulator BclX, B cell lymphoma 2 like, B2CL1_HUMAN, Bcl 2 like 1 protein, Bcl X, Bcl xL, BCL XL/S, Bcl xS, Bcl-2-like protein 1, Bcl2 Like 1, Bcl2 related gene, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, DKFZp781P2092, PPP1R52, Protein phosphatase 1 regulatory subunit 52

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Bcl-XL Antibody (A12541)

Western blot analysis of extracts of various cell lines, using Anti-Bcl-XL Antibody (A12541) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Bcl-XL Antibody (A12541)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Bcl-XL Antibody (A12541) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Bcl-XL Antibody (A12541)

Immunofluorescence analysis of C6 cells using Anti-Bcl-XL Antibody (A12541) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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