Recombinant rabbit monoclonal [DM4] antibody to BCMA.
Applications
ELISA, Flow Cytometry, IF, IP
Dilutions
Flow Cytometry: 1:100, IP: 1:30
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM4
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze / thaw cycles.
Synonyms
B cell maturation antigen, B cell maturation factor, B cell maturation protein, B-cell maturation protein, BCM, CD269, CD269 antigen, TNFRSF17, TNR17_HUMAN, Tumor necrosis factor receptor superfamily member 17
A. Flow cytometry analysis with Anti-BCMA Antibody [DM4] - Azide free (A318679) on K562-BCMA (red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (negative control cell line) (blue histogram). B. Flow cytometry data of serially titrated Anti-BCMA Antibody [DM4] - Azide free (A318679). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with FLAG tagged human BCMA full length gene) were pre-incubated with six different rabbit monoclonal Anti-BCMA Antibodies, including Anti-BCMA Antibody [DM4] - Azide free (A318679), and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with Mouse Anti-FLAG Tag Antibody.
A. Flow cytometry analysis with Anti-BCMA Antibody [DM4] - Azide free (A318679) on NCI-H929 cells (red histogram) or rabbit control antibody on NCI-H929 cells (blue histogram). B. Flow cytometry data of serially titrated Anti-BCMA Antibody [DM4] - Azide free (A318679) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different monoclonal antibodies by titration of antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various Anti-BCMA Antibodies were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4°C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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