This antibody recognises the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.
Applications
WB
Dilutions
WB: 1:200
Reactivity
Human, Mouse, Rat
Immunogen
A peptide corresponding to the N-terminus human actin beta.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
200 µg/ml
Molecular Weight
Aproximately 42 kDa when run under reducing conditions
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.2% Gelatin and 0.1% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti actin beta antibody recognizes the product of the ACTB gene, cytoplasmic actin beta, also known as actin cytoplasmic and beta actin1. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and re-modeling.Actin beta is highly conserved throughout evolution, with all vertebrate and invertebrate species reviewed to date bearing a 98% or greater amino acid primary sequence identity to human actin beta. Plant species also exhibit a high degree of primary sequence conservation displaying considerable similarity to animal actin beta. For example, nearly 90% sequence identity is observed between human ACTB and the model plant species Arabidopsis thaliana (mouse eared cress) ACT1 (Arabidopsis) gene products. Actin beta is a commonly used western blotting loading control.
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