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Anti-beta Actin Antibody [BA3R] (A85272)

This product is identical to Thermo (MA5-15739). Discover more for less.

Western Blot - Anti-ß-Actin Antibody (A85272) - Antibodies.com Western Blot - Anti-ß-Actin Antibody (A85272) - Antibodies.com
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for "beta Actin Antibody"
$195
Product Datasheet

Mouse monoclonal (BA3R) antibody to beta Actin for Dot, ELISA, IS and WB.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-beta Actin Antibody [BA3R]
Description
Mouse monoclonal (BA3R) antibody to beta Actin.
Specificity
Recognizes native and denatured forms of ß-Actin (~42kDa).
Applications
Dot, ELISA, IS, WB
Reactivity
Chicken, Human, Mouse, Rat, Rabbit
Immunogen
ß-Actin N-terminal peptide-KLH conjugates.
Host
Mouse
Clonality
Monoclonal
Clone ID
BA3R
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Protein A affinity chromatography from mouse ascites fluid.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline, pH 7.20, with 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
A26C1A, A26C1B, ACTB, ACTB_HUMAN, Actin beta, Actin cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-ß-Actin Antibody (A85272) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-beta Actin Antibody [BA3R] (A85272)
Three of Antibodies.com loading control mAbs reacting against 10µg/lane of mouse brain tissue lysates. 50kDa band is Anti-ß-Tubulin (BT7R) at 1:2000 dilution (0.5µg/mL); 42kDa band is Anti-ß-Actin (BA3R) at 1:1000 dilution (1µg/mL); 37kDa band is Anti-GAPDH (GA1R) at 1:5000 dilution (0.2µg/mL).
Western Blot - Anti-ß-Actin Antibody (A85272) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-beta Actin Antibody [BA3R] (A85272)
1:1000 (1µg/mL) Ab dilution used in WB of 20µg/lane tissue lysates from human (1), mouse (2), rat (3), and rabbit (4).
Publishing research using Anti-beta Actin Antibody [BA3R] (A85272)? Please let us know so that we can list the citation on this page.

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