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Anti-beta Galactosidase Antibody (A257)

This product is identical to Abcam (ab221199). Discover more for less.

Western blot analysis of beta galactosidase in E. coli crude extract. Immunoprecipitation of beta galactosidase from E. coli crude extract. Immunofluorescence staining of beta galactosidase expressed in HEK293A cells. HEK293A cells were transfected with beta-Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with Anti-beta Galactosidase Antibody (1:500) and Anti-Rabbit IgG (Alexa 555) (1/500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody only reacts with transfected cells. Titration of antibody reactivity of Anti-beta Galactosidase Antibody by ELISA. Plate was coated with 100ng of beta galactosidase per well and 100µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG (HRP) was added as secondary antibody. Color was developed with TMB as substrate.
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for "beta galactosidase Antibody"
$370

Rabbit polyclonal antibody to beta Galactosidase for WB, IP, ICC/IF and ELISA.

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Freight Charges
$40 to United States
Lead Time
4-6 Business Days

Telephone
+1 (314) 370-6046
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Name
Anti-beta Galactosidase Antibody
Description
Rabbit polyclonal antibody to beta Galactosidase.
Applications
WB, IP, ICC/IF, ELISA
Dilutions
WB: 1:1,000-1:2,000, IP: 1:200-1:500, ICC/IF: 1:200-1:500, ELISA: 1:2,000-1:3,000
Reactivity
Escherichia coli beta Galactosidase and beta Gal Tagged proteins.
Immunogen
Full length E. coli beta Galactosidase.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
2 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Acid beta galactosidase, Beta D galactosidase, Beta gal, Beta galactosidase 1, EBP, ECK0341, ELNR1, Galactosidase beta 1, GLB1, JW0335, Lactase, lacZ, MPS4B
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western blot analysis of beta galactosidase in E. coli crude extract.Enlarge Image
Figure 1: Anti-beta Galactosidase Antibody (A257)
Anti-beta Galactosidase Antibody
Immunoprecipitation of beta galactosidase from E. coli crude extract.Enlarge Image
Figure 2: Immunoprecipitation - Anti-beta Galactosidase Antibody (A257)
Anti-beta Galactosidase Antibody
Immunofluorescence staining of beta galactosidase expressed in HEK293A cells. HEK293A cells were transfected with beta-Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with Anti-beta Galactosidase Antibody (1:500) and Anti-Rabbit IgG (Alexa 555) (1/500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody only reacts with transfected cells.Enlarge Image
Figure 3: Immunofluorescence - Anti-beta Galactosidase Antibody (A257)
Anti-beta Galactosidase Antibody
Titration of antibody reactivity of Anti-beta Galactosidase Antibody by ELISA. Plate was coated with 100ng of beta galactosidase per well and 100µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG (HRP) was added as secondary antibody. Color was developed with TMB as substrate.Enlarge Image
Figure 4: Anti-beta Galactosidase Antibody (A257)
Anti-beta Galactosidase Antibody
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