Anti-beta III Tubulin Antibody [ARC0456] (A309019)

Name: Anti-beta III Tubulin Antibody [ARC0456]
Brand: Antibodies.com
SKU: A309019
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0456] antibody to beta III Tubulin for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Cytoskeleton Marker Microtubule Marker Neuronal Marker

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Anti-beta III Tubulin Antibody [TU-20]

Mouse monoclonal [TU-20] antibody to beta III Tubulin for Flow Cytometry (Intracellular), WB, IHC-P and ICC.

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A86691

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Anti-beta III Tubulin Antibody [TU-20]

Mouse monoclonal [TU-20] antibody to beta III Tubulin for Flow Cytometry (Intracellular), WB, IHC-P and ICC.

Specifications

Name

Anti-beta III Tubulin Antibody [ARC0456]

Description

Rabbit monoclonal [ARC0456] antibody to beta III Tubulin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 351-450 of human ßIII-Tubulin/ß3-Tubulin (Q13509).

Sequence

VAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYEDDEEESEAQGPK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0456

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

beta 3 tubulin, beta-4, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, M(beta)3, M(beta)6, MC1R, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, QccE-11995, QccE-15186, TBB3_HUMAN, Tubb 3, TUBB3, TUBB4, Tubulin beta 3, Tubulin beta 3 chain, Tubulin beta 4, Tubulin beta III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-beta III Tubulin Antibody [ARC0456] (A309019)

Western blot analysis of extracts of various cell lines, using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunofluorescence - Anti-beta III Tubulin Antibody [ARC0456] (A309019)

Immunofluorescence analysis of SH-SY5Y cells using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-beta III Tubulin Antibody [ARC0456] (A309019)

Immunofluorescence analysis of mouse brain using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-beta III Tubulin Antibody [ARC0456] (A309019)

Immunofluorescence analysis of rat brain using Anti-beta III Tubulin Antibody [ARC0456] (A309019) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).