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Anti-beta Tubulin Antibody [4E4] (A85429)

Immunofluorescence - Anti-Beta Tubulin Antibody (A85429) - Antibodies.com Western Blot - Anti-Beta Tubulin Antibody (A85429) - Antibodies.com Western Blot - Anti-Beta Tubulin Antibody (A85429) - Antibodies.com
$185
Product Datasheet

Mouse monoclonal (4E4) antibody to beta Tubulin for WB, ICC/IF and IHC.

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A86730
Anti-beta Tubulin Antibody [TU-13]
Mouse monoclonal [TU-13] antibody to beta Tubulin for WB, IHC-Fr and ICC.
Name
Anti-beta Tubulin Antibody [4E4]
Description
Mouse monoclonal (4E4) antibody to beta Tubulin.
Specificity
This antibody reacts with recombinant beta Tubulin but not recombinant alpha Tubulin by ELISA and dot blots.
Applications
WB, ICC/IF, IHC
Dilutions
WB: 1:5,000-1:10,000, ICC/IF: 1:1,000-1:5,000, IHC: 1:1,000-1:5,000
Reactivity
Human, Monkey, Rat, Mouse
Immunogen
Pig brain tubulin preparation.
Host
Mouse
Clonality
Monoclonal
Clone ID
4E4
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescence - Anti-Beta Tubulin Antibody (A85429) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-beta Tubulin Antibody [4E4] (A85429)
HeLa cells stained with Anti-Beta Tubulin Antibody (green), Anti-Lamin A/C Antibody (A85443 | red) and DNA (DAPI | blue). The Anti-Beta Tubulin Antibody reveals strong microtubular staining in the cytoplasm of HeLa cells, while the Lamin A/C localizes in the nuclear membrane.
Western Blot - Anti-Beta Tubulin Antibody (A85429) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-beta Tubulin Antibody [4E4] (A85429)
Western blot analysis of equal amounts of cell line and whole brain lysates using Anti-Beta Tubulin Antibody (1:5,000 | green): [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] NIH-3T3, [7] C6 cells, [8] rat brain, and [9] mouse brain. Strong band at 50 kDa corresponds to the ß-tubulin proteins.
Western Blot - Anti-Beta Tubulin Antibody (A85429) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-beta Tubulin Antibody [4E4] (A85429)
Blot of HeLa cell lysates blotted with Anti-Beta Tubulin Antibody. Note the single sharp clean band corresponding to ß-tubulin at 55 kDa.
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