Western Blot - Anti-beta Tubulin Antibody [AMC0498] (A80389)
Western blot analysis of extracts of various cell lines, using Anti-beta Tubulin Antibody [AMC0498] (A80389) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-beta Tubulin Antibody [AMC0498] (A80389)Immunohistochemistry analysis of paraffin-embedded rat ovary using beta-Tubulin Mouse mAb (AC021) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-beta Tubulin Antibody [AMC0498] (A80389)Immunohistochemistry analysis of paraffin-embedded rat stomach using beta-Tubulin Mouse mAb (AC021) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-beta Tubulin Antibody [AMC0498] (A80389) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH-3T3 cells using Anti-beta Tubulin Antibody [AMC0498] (A80389) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U-2 OS cells using Anti-beta Tubulin Antibody [AMC0498] (A80389) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).