Anti-BIG-2 Antibody [ARC2761] (A305779)

Name: Anti-BIG-2 Antibody [ARC2761]
Brand: Antibodies.com
SKU: A305779
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC2761] antibody to BIG-2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-BIG-2 Antibody [ARC2761]

Description

Rabbit monoclonal [ARC2761] antibody to BIG-2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human CNTN4 (Q8IWV2).

Sequence

EPTKPPASIFARSLSATDIEVFWASPLEKNRGRIQGYEVKYWRHEDKEENARKIRTVGNQTSTKITNLKGSVLYHLAVKAYNSAGTGPSSATVNVTTRKPP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2761

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

135 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AXCAM, Axonal associated cell adhesion molecule, BIG 2, Brain derived immunoglobulin superfamily protein 2, Brain-derived immunoglobulin superfamily protein 2, CNTN4, CNTN4A, CNTN4_HUMAN, contactin 4, Contactin-4, Neural cell adhesion protein BIG 2, SCA16

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-BIG-2 Antibody [ARC2761] (A305779)

Western blot analysis of extracts of various cell lines, using Anti-BIG-2 Antibody [ARC2761] (A305779) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-BIG-2 Antibody [ARC2761] (A305779)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-BIG-2 Antibody [ARC2761] (A305779) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-BIG-2 Antibody [ARC2761] (A305779)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-BIG-2 Antibody [ARC2761] (A305779) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-BIG-2 Antibody [ARC2761] (A305779)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-BIG-2 Antibody [ARC2761] (A305779) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.