Anti-BNIP3 Antibody [ARC50531] (A305317)

Name: Anti-BNIP3 Antibody [ARC50531]
Brand: Antibodies.com
SKU: A305317
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC50531] antibody to BNIP3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-BNIP3 Antibody [ARC50531]

Description

Rabbit monoclonal [ARC50531] antibody to BNIP3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human BNIP3 (NP_004043.3).

Sequence

AFPPPAAEAHPAARRGLRSPQLPSGAMSQNGAPGMQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50531

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

22 - 28 kDa / 50 - 55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCL2 Adenovirus E1B 19kDa Interacting Protein 3, BCL2/adenovirus E1B 19 kDa protein interacting protein 3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP 3, BNIP3_HUMAN, NIP 3, NIP3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-BNIP3 Antibody [ARC50531] (A305317)

Western blot analysis of extracts of various cell lines, using Anti-BNIP3 Antibody [ARC50531] (A305317) at 1:1,000 dilution. Hela cells and NIH/3T3 cells were treated by Cobalt chloride (0. 1 mM) at 37°C for 4 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-BNIP3 Antibody [ARC50531] (A305317)

Immunohistochemistry analysis of paraffin-embedded human cervix cancer tissue using Anti-BNIP3 Antibody [ARC50531] (A305317) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-BNIP3 Antibody [ARC50531] (A305317)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-BNIP3 Antibody [ARC50531] (A305317) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-BNIP3 Antibody [ARC50531] (A305317)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-BNIP3 Antibody [ARC50531] (A305317) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.