Anti-Bovine IgM Antibody [IL-A30] (A280730)

Anti-Bovine IgM Antibody [IL-A30]

Mouse monoclonal [IL-A30] antibody to Bovine IgM.

This antibody recognises bovine IgM, expressed by bovine B-cells. Clone IL-A30 reacts with both serum IgM and cell surface IgM expressed by B lymphocytes. recognises a monomorphic epitope within bovine IgM. The percentage of IgM+ve lymphocytes may vary widely ,ranging between 4 - 30% , within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals.

IP, Flow Cytometry, ELISA, RIA

ELISA: 1:500 - 1:5,000, Flow Cytometry: 1:25 - 1:50, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine

Bovine peripheral blood mononuclear cells depleted of T lymphocytes.

Mouse

Monoclonal

IL-A30

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine IgM, clone IL-A30 recognizes bovine IgM, expressed by bovine B-cells. Clone IL-A30 reacts with both serum IgM and cell surface IgM expressed by B lymphocytes. Mouse anti Bovine IgM, clone IL-A30 recognizes a monomorphic epitope within bovine IgM (Naessens et al. 1988).The percentage of IgM+ve lymphocytes may vary widely ,ranging between 4 - 30%, within populations of healthy cattle, however the percentage of positive cells remains relatively constant within individual animals (Naessens et al. 1988).