Bovine alpha Lactalbumin ELISA Kit (A87162)

Bovine alpha Lactalbumin ELISA Kit

Bovine alpha Lactalbumin ELISA Kit is a 60 minute competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine alpha Lactalbumin in serum, plasma, tissue homogenates, and other biological fluids.

Competitive (quantitative)

Bovine alpha Lactalbumin ELISA Kit (A87162) is a 1 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of bovine alpha Lactalbumin in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for alpha Lactalbumin. During the assay, alpha Lactalbumin present in the standards or samples competes with HRP-labelled alpha Lactalbumin for binding to the immobilized capture antibody. Following incubation, unbound components are removed by washing and TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of alpha Lactalbumin in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

1.875 ng/mL

1 hour

Bovine

Specific for alpha Lactalbumin with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Alpha-lactalbumin, Lactose synthase B protein, LALBA, Lysozyme-like protein 7, LYZL7

Information online is representative. Always refer to the Product Manual inside the kit.