Anti-BRD2 Antibody (A87828)

Name: Anti-BRD2 Antibody
Brand: Antibodies.com
SKU: A87828
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to BRD2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-BRD2 Antibody

Description

Rabbit polyclonal antibody to BRD2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human BRD2 (NP_005095.1).

Sequence

LLKSLHSAGPPLLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPPRKDLPDSQQQHQSSKKGKLSEQL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BRD 2, BRD2_HUMAN, Bromodomain containing 2, Bromodomain-containing protein 2, D6S113E, DKFZp686N0336, Female sterile homeotic related gene 1, Female sterile homeotic related gene 1, mouse, homolog of, FLJ31942, FSH, FSRG1, KIAA9001, NAT, O27.1.1, Really interesting new gene 3 protein, RING3, RING3 PROTEIN, RNF3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-BRD2 Antibody (A87828)

Western blot analysis of extracts of mouse brian, using Anti-BRD2 Antibody (A87828) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-BRD2 Antibody (A87828)

Western blot analysis of extracts of HeLa cells, using Anti-BRD2 Antibody (A87828) at 1:1,000 dilution. HeLa cells were treated by NaCl (400mM)The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-BRD2 Antibody (A87828)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-BRD2 Antibody (A87828) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-BRD2 Antibody (A87828)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-BRD2 Antibody (A87828) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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