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Anti-Brd4 Antibody (A308056)

Western Blot - Anti-Brd4 Antibody (A308056) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit polyclonal antibody to Brd4 for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Brd4 Antibody
Description
Rabbit polyclonal antibody to Brd4.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 623-722 of human BRD4 (NP_055114.1).
Sequence
NKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFSSSESESSSESSSSDSEDSETGPA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
200 kDa / 140 kDa / 120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BRD4-NUT FUSION, BRD4-NUT fusion oncoprotein, BRD4_HUMAN, Bromodomain containing 4, bromodomain containing protein 4, Bromodomain-containing protein 4, CAP, chromosome associated protein, HUNK1, HUNKI, MCAP, Mitotic chromosome-associated protein, Protein HUNK1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Brd4 Antibody (A308056) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Brd4 Antibody (A308056)
Western blot analysis of extracts from normal (control) and BRD4 Rabbit polyclonal antibody knockout (KO) HeLa cells, using Anti-Brd4 Antibody (A308056) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Publishing research using Anti-Brd4 Antibody (A308056)? Please let us know so that we can list the citation on this page.

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